The Cytomegalovirus (CMV) DNA PCR test for urine is a vital diagnostic tool designed to detect and quantify CMV DNA in urine specimens. It plays a crucial role in diagnosing and managing CMV infections, especially in infants and immunocompromised individuals. The test is particularly beneficial for diagnosing congenital CMV infections in infants under 21 days old, a leading cause of congenital viral infections globally. It also aids in monitoring CMV disease progression and response to antiviral therapy in immunocompromised patients. Utilizing real-time polymerase chain reaction (PCR), the test amplifies specific CMV DNA sequences, providing both qualitative and quantitative results. Urine specimens must be collected in sterile conditions and frozen at -20°C within 8 hours to ensure result accuracy. The test's quantitative range is 250 to 1,000,000 copies/mL, essential for assessing disease severity and treatment efficacy. Despite its limitations, the CMV DNA PCR test remains indispensable in viral diagnostic protocols.
The Cytomegalovirus (CMV) DNA PCR test for urine is a vital diagnostic tool designed to detect and quantify CMV DNA in urine specimens. It plays a crucial role in diagnosing and managing CMV infections, especially in infants and immunocompromised individuals. The test is particularly beneficial for diagnosing congenital CMV infections in infants under 21 days old, a leading cause of congenital viral infections globally. It also aids in monitoring CMV disease progression and response to antiviral therapy in immunocompromised patients. Utilizing real-time polymerase chain reaction (PCR), the test amplifies specific CMV DNA sequences, providing both qualitative and quantitative results. Urine specimens must be collected in sterile conditions and frozen at -20°C within 8 hours to ensure result accuracy. The test's quantitative range is 250 to 1,000,000 copies/mL, essential for assessing disease severity and treatment efficacy. Despite its limitations, the CMV DNA PCR test remains indispensable in viral diagnostic protocols.
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The CMV DNA PCR test for urine is essential for diagnosing and managing CMV infections, especially in infants and immunocompromised individuals. Key points include:
- Purpose: Detects and quantifies CMV DNA in urine, aiding diagnosis and management.
- Methodology: Uses real-time PCR to amplify and quantify CMV DNA.
- Specimen Requirements: Urine collected in sterile conditions, frozen at -20°C within 8 hours.
- Applications: Diagnoses congenital CMV in infants; monitors disease in immunocompromised patients.
- Limitations: Negative results don't rule out infection; false negatives possible.
- Turnaround: Results in 2-4 days, aiding timely clinical decisions.
The CMV IgM antibody test is crucial for diagnosing recent CMV infections. It detects IgM antibodies, indicating a current or recent infection. However, a negative result doesn't entirely rule out infection, especially in early stages or mild cases. Key considerations include:
- Primary Infection: IgM may not be detectable early on, affecting diagnosis in infants and pregnant women.
- Reactivation: Positive results can indicate reactivation, common in transplant patients.
- Immunocompromised Patients: They may show delayed seroconversion, complicating diagnosis.
Clinical correlation is essential, and additional testing may be needed for accurate diagnosis.
Understanding test results is essential for effective diagnosis and management of CMV infections. Here's a brief guide:
- Positive Result: Indicates presence of CMV DNA. Requires clinical correlation for treatment decisions.
- Negative Result: Does not rule out infection. Consider clinical context and potential false negatives.
- Quantitative Data: Provides CMV DNA levels, aiding in assessing infection severity and treatment efficacy.
- Clinical Context: Results should be interpreted alongside patient history and other tests for accurate diagnosis.
- Limitations: Be aware of potential false negatives and the influence of viral prevalence on predictive value.
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