Monospot

The Monospot test is a diagnostic tool used to detect infectious mononucleosis (IM), commonly caused by the Epstein-Barr virus (EBV). It identifies heterophile antibodies in the blood, which are produced in response to EBV infection. The test involves a latex agglutination process using equine erythrocytes, where the presence of these antibodies causes clumping, indicating a positive result. Typically, a blood sample is collected via venipuncture or fingerstick, and results are available within 5 to 10 minutes. The test is indicated for patients with symptoms like sore throat, fever, and fatigue, which overlap with other viral illnesses. However, its sensitivity ranges from 70 to 90%, and it may not detect all IM cases, especially in young children or heterophile-negative cases. While the test has high specificity, false positives can occur due to other conditions. Additional EBV-specific antibody tests may be needed for confirmation in cases of negative Monospot results with persistent symptoms.

Price of test:£53

The lowest price from the last 30 days: £53

Awaiting result:

12 hours

Collect material:

Blood

Prepare:

Learn more

Purpose of the test

The Monospot test is a diagnostic tool for detecting infectious mononucleosis (IM), primarily caused by the Epstein-Barr virus (EBV). It identifies heterophile antibodies in the blood, indicating EBV infection. Key points include:

- Procedure: Blood sample collection, results in 5-10 minutes.
- Indications: Symptoms like sore throat, fever, and fatigue.
- Limitations: Sensitivity of 70-90%, less effective in young children.
- Interpretation: Positive indicates IM; negative may require further testing.
- Specificity: High, but false positives possible.
- Clinical Use: Common in clinics, but CDC does not endorse for general use.
- Additional Testing: EBV-specific antibody tests for confirmation.

Who Should Get Tested

The Monospot test is crucial for diagnosing infectious mononucleosis (IM), primarily caused by the Epstein-Barr virus (EBV). It detects heterophile antibodies in the blood, indicating EBV infection. Here's who should be tested:

- Symptomatic Patients: Those with sore throat, fever, malaise, tonsillitis, lymphadenopathy, and fatigue.
- Prolonged Symptoms: Individuals with symptoms persisting beyond typical viral infections.
- Exclusion of Other Illnesses: When other conditions like hepatitis or lupus are suspected.

Despite its utility, the test's sensitivity varies, necessitating further testing if results are negative but symptoms persist.

Blood Test Preparation Guidelines

Time of day

It is recommended to schedule your appointment for blood tests in the morning hours between 7:00-10:00.

Fasting

It is recommended to fast for approximately 12 hours before blood sampling. The last meal of the previous day should ideally be consumed around 6:00 p.m. On the day before the test, avoid heavy and fatty meals as well as alcohol.

Stay hydrated

Drinking water prior to testing can help with sample collection.

Other factors

Tests should not be performed after a sleepless night or intense physical activity. It is recommended to avoid exercise and stress immediately before blood collection and to not smoke. A short rest is recommended.

Medications and supplements:

Blood samples should be collected before the morning dose. Some drugs can interfere with test results. Consult with your doctor whether you can delay your dose because of lab tests.

Biotin supplements:

High doses of biotin supplements can affect test results, causing false elevation or reduction. It's recommended to avoid taking biotin for at least 72 hours before blood collection. If you are taking biotin, inform the personnel collecting the blood so that they can provide specific instructions.

Interpreting Test Results

Understanding test results is crucial for accurate diagnosis and management. The Monospot test detects heterophile antibodies to diagnose infectious mononucleosis (IM) caused by the Epstein-Barr virus (EBV). Here's a quick guide to understanding your results:

- Positive Result: Indicates presence of heterophile antibodies, suggesting IM.
- Negative Result: Does not rule out IM, especially if tested early.
- Sensitivity: 70-90%, may miss some cases, particularly in young children.
- Specificity: 95-100%, though false positives can occur with other conditions.
- Further Testing: Consider EBV-specific antibody tests if symptoms persist despite negative results.

Price of test:£53

The lowest price from the last 30 days: £53

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