- Main page
- Tests catalogue
- Wheat IgE
Wheat IgE
Whipple's disease is a rare systemic infection caused by the bacterium *Tropheryma whipplei*, primarily affecting the small intestine but potentially involving other body parts, leading to symptoms like malabsorption, weight loss, diarrhea, joint pain, and neurological issues. Diagnosing Whipple's disease requires a combination of clinical evaluation, laboratory tests, and histopathological examination. A key diagnostic tool is the Polymerase Chain Reaction (PCR) test, which detects *Tropheryma whipplei* DNA in clinical specimens with high sensitivity and specificity. Specimens suitable for PCR testing include whole blood, cerebrospinal fluid, tissue samples, saliva, and stool. The PCR process involves DNA extraction, amplification using specific primers, and detection through real-time PCR assays. While PCR is highly effective, it has limitations, such as potential contamination and inability to distinguish between active and latent infections. Despite these challenges, PCR remains crucial for diagnosing Whipple's disease and monitoring treatment response.
Whipple's disease is a rare systemic infection caused by the bacterium *Tropheryma whipplei*, primarily affecting the small intestine but potentially involving other body parts, leading to symptoms like malabsorption, weight loss, diarrhea, joint pain, and neurological issues. Diagnosing Whipple's disease requires a combination of clinical evaluation, laboratory tests, and histopathological examination. A key diagnostic tool is the Polymerase Chain Reaction (PCR) test, which detects *Tropheryma whipplei* DNA in clinical specimens with high sensitivity and specificity. Specimens suitable for PCR testing include whole blood, cerebrospinal fluid, tissue samples, saliva, and stool. The PCR process involves DNA extraction, amplification using specific primers, and detection through real-time PCR assays. While PCR is highly effective, it has limitations, such as potential contamination and inability to distinguish between active and latent infections. Despite these challenges, PCR remains crucial for diagnosing Whipple's disease and monitoring treatment response.
The lowest price from the last 30 days: £34
Awaiting result:
One day
Collect material:
Blood
Prepare:
Whipple's disease diagnosis hinges on precise laboratory techniques. PCR is pivotal, amplifying *Tropheryma whipplei* DNA for detection. Key specimen types include:
- Whole Blood: Use lavender-top (EDTA) tubes; store refrigerated or frozen.
- Cerebrospinal Fluid: Transfer 1 mL to a sterile container.
- Tissue: Collect fresh tissue in sterile containers; refrigerate or freeze.
- Saliva and Stool: Useful when other samples aren't available.
PCR involves DNA extraction, amplification, and detection, ensuring high sensitivity and specificity. Despite its utility, PCR's limitations necessitate careful specimen handling to avoid contamination.
The Wheat IgE test is essential for diagnosing wheat allergies, which are common food allergies. It identifies IgE antibodies in the serum, indicating an immune response to wheat allergens. Key points include:
- Purpose: Diagnoses wheat allergy, confirms sensitization, and investigates allergic reactions.
- Procedure: Serum sample collection, using serum gel or red-top tubes.
- Specimen Stability: Refrigerated for 14 days or frozen for 90 days.
- Methodology: Uses Fluorescence Enzyme Immunoassay (FEIA).
- Interpretation: Positive results suggest IgE-mediated sensitivity.
- Management: Avoid wheat, use medications, and consider immunotherapy.
Understanding test results for Whipple's disease involves interpreting PCR findings, which detect *Tropheryma whipplei* DNA. Key points include:
- Positive Result: Indicates the presence of the bacterium, confirming infection.
- Negative Result: Does not rule out infection; may result from PCR inhibition or low DNA levels.
- Clinical Context: Results should be considered alongside clinical evaluation and other tests.
- Monitoring: PCR can track treatment efficacy by showing changes in bacterial load.
- Limitations: Contamination and inability to distinguish active from latent infections can affect accuracy.
The lowest price from the last 30 days: £34
What is GetLabTest.com?
How do I get started with GetLabTest.com?
What diseases can GetLabTest.com detect?
How do I prepare for testing with GetLabTest.com?
Can I speak with a doctor about my results?
Is testing with GetLabTest.com covered by insurance?
How long does it take to receive the test results?
Can I access my test results online?
Is my personal information kept confidential?
What if I have questions or concerns about my test results?